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                                霍乱毒素B亚单位

                                霍乱毒素B亚基、CHOLERA TOXIN B SUBUNIT、CTB、 Cholera Toxin B subunit、CTxB、Choleragenoid

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                                货号 abs80001
                                规格 价格
                                ¥ 2200
                                库存?#21050;?/span>现货

                                数量:

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                                产品介绍 商品评论 引用文献(12)
                                概述
                                描述

                                Cholera toxin (CTB,霍乱毒素B亚基)belongs to the AB5 –subunit family oftoxins.The native hexameric protein has a molecular mass of ~85 kDa and contains two subunits. It consists of a single A subunit (~27.2 kDa), responsible for the ADP-ribosylation activity, and five B subunits(~11.6 kDa each), which are arranged as a pentameric ring with an apparent 5-fold symmetry and are associated with the cell surface receptor binding and subsequent internalization (transmembrane transport)of the enzymatic component.

                                别名 霍乱毒素B亚基、CHOLERA TOXIN B SUBUNIT、CTB、 Cholera Toxin B subunit、CTxB、Choleragenoid
                                性能
                                保存方法 2-8 oC
                                产品用途 Cholera toxin and its purified A subunit are frequently used for the study of signal transduction mechanisms.In addition,cholera toxin acts as an adjuvant through the stimulation of B lymphocytes. The cholera toxin B subunit alone is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
                                纯度 ≥95%
                                分子量 11kd
                                溶解性 H2O: soluble 1mg/mL
                                CAS号 131096-89-4
                                外观 power
                                化学性质 2–8 °C, The product, as supplied, is stable 2 years when stored properly. Solutions
                                are stable for at least 3 months when stored at 2–8 °C. DO NOT FREEZE.
                                技术指标 Format:Recombinant Source:E.coli Sodium azide:Not add.
                                声明

                                The B subunit is non-toxic to cells and possesses no intrinsic adenylate cyclase activity. The cholera toxin B subunit (CTB) attaches to cells by binding to ganglioside GM1.8 CTB has been shown to be a good label for microglial cells (due to the enrichment of ganglioside GM1 on their cell surface), but not for oligodendrocytes or astrocytes. The B subunit has been reported to be an excellent tracer for the study of axonal transport using immunohistochemical methods. Recently it has been widely used as a marker of membrane lipid rafts, which are membrane microdomains enriched with cholestrol and sphingolipids. These lipid rafts have an important role in cell signaling and protein trafficking.

                                参考文献
                                1. Nedelkoska, L., and Benjamins, J.A., Binding of cholera toxin B subunit: a surface marker for murine microglia but not oligodendrocytes or astrocytes. J. Neurosci. Res., 53, 605-612 (1998).
                                2. Janes, P.W., et al., Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor. J. Cell Biol., 147, 447-461 (1999).
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